The tricky thing about dressing for Knoxville in April is the temperature swing. You'll start at 7:30 AM when it might be 48 degrees and dewy, and finish sometime between 10:30 AM and 1:30 PM when it could be 68 and sunny. That's a 20-degree range across a single race, and what feels perfect at the start line will feel wrong by mile 18.
The rule for this race: dress for how you'll feel at mile 16, not mile 1.
This is the ideal scenario and your simplest outfit. Short-sleeve tech shirt, running shorts, and that's it. You'll be slightly chilly in the corral, which is exactly right. By mile 3 you'll be warm. Light gloves and a throwaway long-sleeve for the start are reasonable if you run cold, but you'll shed them by the time you hit Neyland Drive.
Singlet or the lightest tech shirt you own. Short shorts. A light-colored hat or visor to block the sun. Sunglasses. Sunscreen, applied before you leave the hotel, not in the corral. On a warm Knoxville day, managing heat is your primary clothing concern, and every unnecessary layer costs you.
Knoxville has had rainy race days, and April thunderstorms are a real possibility. A light waterproof cap is more useful than a rain jacket. Running in a rain jacket for 26.2 miles creates a greenhouse effect that's worse than just being wet. If it's rainy and cold (below 55 with rain), a thin, breathable windbreaker over a tech shirt is acceptable, but you should have tested this combination in training.
Even on a day that looks moderate (mid-60s, partly cloudy), if humidity is above 65%, you will sweat more and cool less efficiently than the temperature suggests. Dress lighter than you think you need to. Moisture-wicking everything. No cotton, anywhere, for any reason.
Unlike most marathons, Knoxville explicitly allows headphones. That said, there are 24 live musical acts on the course, and the neighborhood support sections (Sequoyah Hills, Island Home) are part of what makes this race special. Consider running at least some miles with the volume low. A coaching app like PaceKit uses your Apple Watch speaker, so you don't need headphones at all.
The short version: Singlet or light tech shirt, shorts, sunscreen, and a hat. Knoxville is more likely to be too warm than too cold on race day, and overdressing is a more common mistake than underdressing.